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تاثیر زمان و ترکیب محیط کشت بر تولید IAA توسط جدایه های مختلف سودوموناس فلورسنت و تاثیر جدایه ها بر رشد ذرت (Zea mays L) | ||
تحقیقات کاربردی خاک | ||
مقاله 2، دوره 2، شماره 2، بهمن 1393، صفحه 2-27 اصل مقاله (351.35 K) | ||
نوع مقاله: مقاله پژوهشی | ||
نویسندگان | ||
دیناالسادات رضایی1؛ پیمان عباس زاده دهجی* 2؛ عبدالرضا اخگر3؛ علی اشرف سلطانی4 | ||
1دانشجوی کارشناسی ارشد گروه علوم خاک دانشگاه ولیعصر رفسنجان | ||
2استادیار گروه علوم خاک دانشگاه ولیعصر رفسنجان (مکاتبه کننده) | ||
3دانشیار گروه علوم خاک دانشگاه ولیعصر رفسنجان | ||
4استادیار گروه علوم و مهندسی خاک دانشکده فناوری کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی | ||
چکیده | ||
باکتریهای سودوموناس فلورسنت از مهمترین باکتریهای محرک رشد گیاه در ریزوسفر گیاهان مختلف زراعی میباشند که با داشتن خصوصیات محرک رشدی متعدد، میتوانند موجب بهبود رشد گیاه گردند. تولید غلظتهای بالای IAA یکی از ویژگیهای بارز برای اکثر این باکتریها است. بهمنظور بررسی توانایی تولید اکسین توسط سویههای مختلف باکتری سودوموناس فلورسنت در زمانهای متفاوت و در محیطهای کشت TSB و DF و همچنین تعیین تاثیرات این سویهها بر شاخصهای رشد گیاه ذرت، آزمایشی در قالب طرح کاملا تصادفی اجرا گردید. نتایج نشاندهنده توانایی تمام سویهها، در تولید IAA بود. متوسط میزان تولید IAA در محیط کشت TSB بین 040/0 تا 3/34 میلیگرم بر لیتر متغیر بود. سویههای13p و 26p در روز پنجم بهترتیب کمترین و بیشترین میزان IAA را تولید نمودند. در محیط کشت DF نیز متوسط تولید IAA توسط جدایهها بین 043/0 تا 03/6 میلیگرم بر لیتر متغیر بود. همبستگی معنیدار بین اکسین تولید شده در دو محیط کشت در روزهای مختلف، نشان داد که روند تولید اکسین توسط جدایهها در هر دو محیط کشت یکسان است و جدایههایی که توان بالایی در تولید اکسین دارند در هر دو محیط مقادیر بالاتری اکسین در مقایسه با جدایههای ضعیف تولید کردند. نتایج آزمون گلخانهای نیز نشان داد که تلقیح جدایههای مورد استفاده، وزن خشک اندام هوایی را تا بیش از 100 درصد و طول اندام هوایی، سطح برگ و کلروفیل را بهترتیب 1/53، 2/57، 3/22 درصد افزایش دادند. کاربرد اکثر جدایهها سبب کاهش وزن خشک ریشه گردید. نتایج نشاندهندهی وجود همبستگی مثبت و معنیدار بین تولید IAA در دو محیط غنی TSB و حداقل DF بود. این همبستگی بیانگر این است که در انتخاب جدایههای برتر میتوان از هر دو محیط کشت استفاده کرد. | ||
کلیدواژهها | ||
اکسین؛ ذرت؛ حجم ریشه؛ سطح برگ؛ سودوموناس فلورسنت | ||
مراجع | ||
References
Abbaszade-Dahaji P, Asadi-Rhmani H, Saleh-Rastin N, Khvazi K and Soltani AA. 2009. Evaluation of auxin production by fluorescent Pseudomonas and their effects on seedling growth of canola (Brassica napus L). Iranian Journal of Soil and Water Science, 22(2): 203-215. (in Persian). Ahmad F, Ahmad I and Khan MS. 2008. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiological Research, 163(2): 173-181. Akhtar S and Ali B. 2011. Evaluation of rhizobacteria as non-rhizobial inoculants for mung beans. Australian Journal of Crop Science, 5(13): 1723-1729. Alipour ZT and Sobhanipour A. 2012. The effect of Thiobacillus and Pseudomonas fluorescent inoculation on maize growth and Fe uptake. Annals of Biological Research,3 (3): 1661-1666. Almaghrabi OA, Abdelmoneim TS, Albishri HM and Moussa TAA. 2014. Enhancement of maize growth using some plant growth promoting rhizobacteria (PGPR) under laboratory conditions. Life Science Journal, 11(11): 764-772. Antoun H, Beauchamp CJ, Goussard N, Chabot R and Lalande R. 1998. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: Effect on radishes (Raphanus sativus L.). Plant and Soil, 204(1): 57–67. Arshad M and Frankenberger WT. 1991. Microbial production of plant hormones. Plant and Soil, 133: 1-8. Bashan Y and de-Bashan LE. 2005. Plant growth-promoting. In: Encyclopedia of soils in the environment. Hillel D. Elsevier, Oxford, UK. pp: 103-115. Bashan Y, Bustillos JJ, Leyva LA, Hernandez JP and Bacilio M. 2006. Increase in auxiliary photoprotective photosynthetic pigments in wheat seedlimgs induced by Azospirillum brasilence. Biology and Fertility of Soils,42: 279-285. Bent E, Tuzan S, Chanway CP and Enebak S. 2001. Alteration in plant growth and in root hormone levels of lodgepole pines inoculated with rhizobacteria. Canadian Journal of Microbiology, 47: 793-800. Bertrand H, Nalin R, Bally R and Cleyet-Marel JC. 2001 Isolation and identification of the most efficient plant growth promoting bacteria associated with canola brassica napus. Biology and fertility of soils, 33(2): 152-156. Bodelier PL, Wijlhuizen AG, Blom CW and Laanbroek HJ. 1997. Effects of photoperiod on growth of and denitrification by Pseudomonas chlororaphis in the root zone of Glyceria maxima, studied in a gnotobiotic microcosm. Plant and Soil, 190(1): 91–103. Boven GD and Rovira AD. 1999. The rhizosphere and its management to improve plant growth. Advances in Agronomy, 66: 1-102. Chi F, Shen SH, Cheng HP, Jing YX, Yanni YG and Dazzo FB. 2005. Ascending migration of endophytic rhizobia, from roots to leaves, inside rice plants and assessment of benefits to rice growth physiology. Applied and Environmental Microbiology, 71: 7271-7278. Dobbelaere S, Vanderleyden J and Okon Y. 2003. Plant growth-promoting effects of diazotrophs in the rhizosphere, Critical Reviews in Plant Sciences. 22 (2): 107-149. Ebadi A, Alikhani HA and Rashtbari M. 2012. Effect of plant growth promoting bacteria (PGPB) on the morpho-physiological properties of button mushroom agaricus bisporus in two different culturing beds. International Journal of Basic Sciences and Applied Research, 3(1): 203-212. Echeverria SR, Fernandez MAP, Vlaar S and Finnan T. 2003. Analysis of the legume–rhizobia symbiosis in shrubs from central Western Spain. Applied Journal of Microbiology, 95:1367–1374. Gandi A and Sivakumar K. 2010. Impact of vermicompost carrier based bioinoculants on growth, yield and quality of rice (Oryza sativa L). An International Quarterly Journal of Environmental. Science, 83-88. Gholami A, Shahsavani S and Nezarat S. 2009. The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. WorldAcademy of Science Engineering and Technology, 49: 19-24. Hameedaa B, Harinib GO, Rupelab P, Wanib SP and Reddya G. 2008. Growth promotion of maize by phosphate solubilizing bacteria isolated from composts and macrofauna. Microbiological Research, 163: 234-242. Hamidi A, Asgharzadeh A, Chaokan R and Khalvati MA. 2011. Maize (Zea mays L.) seed biofortification by plant growth promoting bacteria (PGPB). International Journal of Agronomy and Plant Production, 2(5): 194-205. Han HS and Lee KD. 2005. Phosphate and potassium solubilizing bacteria effect on mineral uptake, soil availability and growth of Eggplant. Research Journal of Agriculture and Biological Sciences, 1: 176-180. Iqbal A and Hasnain S. 2013. Auxin producing Pseudomonas strains: Biological candidates to modulate the growth of Triticum aestivum beneficially. American Journal of Plant Sciences, 4(9): 1693-1700. Jean JS, Lee SS, Kim HY, Ahn TS, and Song HG. 2003. Plant growth promoting in soil by some inoculated microorganism. Journal of Microbiology, 41(4): 271-276. Karnwal A. 2009. Production of indole acetic acid by fluorescent Pseudomonas in the presence of l-tryptophan and rice root exudates. Journal of Plant Pathology, 91(1): 61-63. Keshavarzi M, Jafari Haghighi B and Bagheri A. 2013. The evaluation of auxin and gibberellin hormone on quantitative and qualitative characteristics of forage corn. Iranian Journal of Plant Ecophysiology, 15: 26-35. (in Persian). Khakipour N, Khavazi K, Mojallali H, Pazira E and Asadi Rahmani H. 2008. Production of auxin hormone by fluorescent pseudomonads. American Eurasian Journal of Agricultural and Environmental Sciences, 4(6): 687–692. Kloepper JW, Lifshitz RR and Zablotwicz RM. 1989. Free-living bacterial inocula for enhancing crop productivity. Trends in Biotechnology, 7: 39-43. Leinhos V, and Vacek O. 1994. Biosynthesis of auxins by phosphate-solubilizing rhizobacteria from wheat and rye. Microbiological Research, 149: 31-35. Leveau JHJ and Lindow SE. 2005. Utilization of the plant hormone Indole-3-acetic acid for growth by Pseudomonas putida strain 1290. Applied and Environmental Microbiology, 71(5): 2365–2371. Lim JH, Ann CH, Kim YH, Jung BK and Kim SD. 2012. Isolation of auxin- and 1-aminocyclopropane-1-carboxylic acid deaminase-producing bacterium and its effect on pepper growth under saline stress. Journal of the Korean Society for Applied Biological Chemistry, 55(5): 607−612. Madhaiyan M, Poonguzhali S, Ryu J and Sa T. 2006. Regulation of ethylene levels in canola (Brassica campestris) by 1-amino cyclopropane- 1-carboxylate deaminase- containing Methylobacterium fujisawaense, Plantarum, 224: 268–278. Maghami M, Olamaee M, Rasouli-Sadaghiani MH and Dordipour E. 2013. Isolation and some characteristics of fluorescent Pseudomonas native plant growth in soybean fields in Golestan province. Iranian Journal of Soil Management and Sustainable Production, 3(2): 251-264 (in Persian). Majeed A, Abbasi MK, Hameed S, Imran A and Rahim N. 2015. Isolation and characterization of plant growth promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Frontiers in Microbiology, 6: 1-10. Mehnaz S, Kowalik T, Reynold B and Lazarovits G. 2010. Growth promoting effects of corn (Zea mays) bacterial isolates under greenhouse and field conditions. Soil Biology and Biochemistry, 42 (10): 1848-1856. Miransari M, Smith DL. 2014. Plant hormones and seed germination. Environmental and Experimental Botany, 99: 110-121. Misko AL and Germida JJ. 2002. Taxonomic and functional diversity of pseudomonad isolated from the roots of field-grown canola. FEMS Microbiology Ecology, 42: 399-407. Montero-Calasanz MC, Santamaria C, Albareda M, Daza A, Duan J, Glick BR and. Camacho M. 2013. Alternative rooting induction of semi-hardwood olive cuttings by several auxin-producing bacteria for organic agriculture systems. Spanish Journal of Agricultural Research, 11(1): 146-154. Noumavo PA, Kochoni E, Didagbe YO, Adjanohoun A, Allagbe M, Sikirou R, Gachomo EW, Kotchoni SO and Baba-Moussa L. 2013. Effect of different plant growth promoting rhizobacteria on maize seed germination and seedling development. American Journal of Plant Sciences, 4(5): 1013-1021. Patten CL and Glick BR. 2002. The role of bacterial indole acetic acid in the development of the host plant root system. Applied and Environmental Microbiology, 68(8): 3795–3801. Penrose DM and Glick BR. 2003. Method for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiologia Plantarum, 18: 10-15. Rubio TMG, Valencia-Plata SA, Bernal-Castillo J and Martinez-Nieto P. 2000. Isolation of Enterobacteria, Azotobacter sp. And Pseudomonas sp. producers of indole- 3-acetic acid and siderophores, from Colombian rice rhizosphere. Revista Latinoamericana de Microbiologia, 42: 171-176 Saharan BS and Nehra V. 2011. Plant growth promoting rhizobacteria: A critical review. Life Science and Medical Research, 21: 1-30 Sarma MVRK, Saharan K, Prakash A, Bisaria VS and Sahai V. 2009. Application of fluorescent pseudomonads inoculant formulations on Vigna mungo through field trial. International Journal of Biological Sciences, 5(1): 25-28. Shaharoona B, Arshad M, ZahirAZ and Khalid A. 2006. Performance of Pseudomonas spp. containing ACC-deaminase for improving growth and yield of maize (Zea mayz L.) In the presence of nitrogenous fertilizer. Soil Biology and Biochemistry, 38: 2971–2975. Spaepen S, Vanderleyden J and Okon Y. 2009. Plant growth-promoting actions of rhizobacteria. In: van Loon LC (ed.) Advances in Botanical Research, Academic, Burlington, pp: 283-320 Tan KZ, Radziah O, Halimi MS, KhairuddinAR, Habib SH and Shamsuddin ZH. 2014. Isolation and characterization of rhizobia and plant growth-promoting rhizobacteria and their effects on growth of rice seedlings. American Journal of Agricultural and Biological Sciences, 9 (3): 342-360. Teale WD, Paponov IA and Palme K. 2006. Auxin in action: Signalling, transport and the control of plant growth and development. Nature Reviews, Molecular Cell Biology, 7(11): 847-59 Tien T, Gaskins M, and Hubbell D. 1979. Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.). Applied and Environmental Microbiology, 37: 1016-1024. Tolay I, Erenoglv B, Romheld V, Braon HJ and CakmakI. 2001. Phytosiderophore release in Aegilops tauschii and triticum species under zinc and iron deficiencies. Journal of Experimental Botany, 52: 1093-1099 Vessey JK and Buss TJ. 2002. Bacillus cereus UW85 inoculation effects on growth, nodulation and N accumulation in grain legumes: Controlled environment studies. Canadian Journal of Microbiology, 82: 283-290. Weyers IDB and Paterson NW. 2001. Plant hormones and the control of physiological processes. New Phytologist, 152: 375-407 ZahirAZ, Arshad M and Frankenbeiger WF. 2004. Plant growth promoting rhizobacteria applications and perspective agriculture. Advances in Agronomy, 81: 97-168. Zhao Y. 2010. Auxin biosynthesis and its role in plant development. Annual Review of Plant Biology, 61: 49–64. | ||
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