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تاثیر کاربری اراضی روی فراوانی نسبی و ترکیب جوامع باکتریایی خاک | ||
تحقیقات کاربردی خاک | ||
مقاله 1، دوره 7، شماره 1، خرداد 1398، صفحه 1-15 اصل مقاله (1005.03 K) | ||
نوع مقاله: مقاله پژوهشی | ||
نویسندگان | ||
قباد جلالی1؛ امیر لکزیان* 2؛ علیرضا آستارایی3؛ علیاکبر حداد مشهد ریزه4؛ مهدی آزادوار5؛ عیسی اسفندیارپور بروجنی6 | ||
1دانشگاه فردوسی مشهد | ||
2عضو هیات علمی گروه علوم خاک-دانشگاه فردوسی مشهد | ||
3عضو هیات علمی گروه علوم خاک- دانشگاه فردوسی مشهد | ||
4عضو هیات علمی گروه زیستشناسی- دانشگاه فردوسی مشهد | ||
5عضو هیات علمی گروه گیاهپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی جنوب استان کرمان | ||
6عضو هیات علمی گروه علوم خاک- دانشگاه ولیعصر (عج) رفسنجان | ||
چکیده | ||
تغییر کاربری اراضی یکی از مهمترین فاکتورهایی بوده که ضمن اثرگذاری روی جوامع ریزجانداران خاک، نقش محوری در اکثر فرآیندهای بیوژئوشیمیایی و اکولوژیکی ایفا میکنند. بهمنظور بررسی تأثیر تغییر کاربری اراضی (از مراتع بوتهزار به کشاورزی) روی فراوانی نسبی و ترکیب جوامع باکتریایی خاک، مطالعهای با استفاده از روش Real-time PCR در سه کاربری اراضی (باغ، زراعی و بوتهزار) در دشت جیرفت انجام شد. در هر کاربری 12 نمونه خاک سطحی از عمق 10 سانتیمتری جمعآوری و برخی از ویژگیهای فیزیکی و شیمیایی آنها اندازهگیری شدند. استخراج و خالصسازی DNA از نمونههای خاک با استفاده از کیت نوکلئواسپین سویل (NucleoSpin® Soil kit) انجام شد. با استفاده از دستگاه Real-time PCR و طبق روش منحنی استاندارد نسبی، مقایسه کمّی غلظت نسبی و دمای ذوب قطعات 16S rDNA باکتریایی خاک بهترتیب برای بررسی فراوانی نسبی و ترکیب جوامع باکتریایی خاک در کاربریهای اراضی مختلف انجام شد. نتایج بررسی غلظت و کیفیت DNA های استخراج شده از نمونههای خاک نشان داد که استخراج و خالصسازی DNA با استفاده از کیت نوکلئواسپین سویل مطلوب بود. نتایج آنالیز واریانس یک طرفه ناپارامتریک کروسکال والیس (در سطح آماری پنج درصد) نشان داد که کاربری اراضی روی فراوانی نسبی و ترکیب جوامع باکتریایی خاک اثر معنیدار داشت؛ بهطوریکه میانگین فراوانی نسبی جوامع باکتریایی خاک در کاربری باغ بیشتر و با دو کاربری دیگر تفاوت معنیدار داشت. همچنین ترکیب جوامع باکتریایی خاک در کاربری بوتهزار با دو کاربری دیگر تفاوت معنیدار داشت. بهطورکلی در دشت جیرفت تغییر کاربری اراضی از بوتهزار به باغ و از بوتهزار به باغ و زراعی بهترتیب سبب افزایش فراوانی نسبی و تغییر معنیدار ترکیب جوامع باکتریایی خاک شده است. | ||
کلیدواژهها | ||
DNA ژنومی خاک؛ 16S rDNA باکتریایی خاک؛ Real-time PCR؛ دشت جیرفت | ||
مراجع | ||
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