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Natural preservation of butter using whey protein hydrolysates: Antioxidant, antimicrobial, and sensory evaluation | ||
| Food Safety and Packaging | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 22 آبان 1404 | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.30466/fsp.2025.56544.1017 | ||
| نویسندگان | ||
| Fatemeh Rezaei* 1؛ Omar Alizadeh2 | ||
| 1Department of Food Hygiene and Industries, Faculty of Paraveterinary Medicine, Ilam University, Ilam, Iran | ||
| 2Veterinary Office of Bukan, Bukan, West Azarbaijan, Iran | ||
| چکیده | ||
| Whey protein hydrolysates (WPHs) are recognized as a valuable source of bioactive peptides with multifunctional properties, especially their potent antioxidant and antimicrobial activities. These characteristics make them attractive natural alternatives to synthetic preservatives and align with the growing consumer demand for clean-label and functional foods. In this study, lyophilized whey protein hydrolysates (LWPH) were incorporated into a butter-based model system to assess their potential as natural preservatives. Four formulations were prepared: (1) control (without additives), (2) butter with 1% (w/w) LWPH, (3) butter with 0.02% (w/w) butylated hydroxytoluene (BHT), and (4) butter with a combination of 1% (w/w) LWPH + 0.02% (w/w) BHT. All samples were stored at 4 ± 1 °C for 60 days, and quality parameters were periodically monitored. Lipid oxidation was determined by the thiobarbituric acid (TBA) assay, and microbial quality was evaluated through total viable count. The results showed that the LWPH + BHT formulation achieved about a 70% reduction in lipid oxidation and a 4 log cycles decrease in total viable count compared with the control. Moreover, the treated samples maintained better overall acceptability throughout storage. These findings indicate that LWPH can serve as effective natural preservatives for extending the shelf life and maintaining the quality of lipid-rich foods such as butter. | ||
| کلیدواژهها | ||
| Butter؛ Hydrolysates؛ Quality attributes؛ Whey protein | ||
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